Effects of folk wisdom boiled Herb extract, Phyllanthus amarus Schum&Thorn, Phyllanthus acidus (L.) Skeels, and pandanus amaryllifolius on neutrophil, red blood cell, and platelet functions

Supaporn Khamchum, Pareeya Baiubol, Nungruthai Nilsri

Abstract


Introduction: Hematopoietic cells consist of red blood cells, white blood cells and platelets. Blood cells play the major roles in oxygen transportation, immune system, and hemostasis, respectively. An imbalance of cell function may lead to many diseases such as hemolytic anemia, immunodeficiency, and thrombosis. Folk wisdom boiled herbs, the traditional medicine from Ban Tontakean, Maejai District, Phayao Province, Thailand, have been used for nourishment and hypertension treatments to study the effect on the hematologic system for pharmacological properties.

Objectives: To study the effects of folk wisdom boiled herb, Phyllanthus amarus Schum&Thorn, Phyllanthus acidus (L.) Skeels, and Pandanus amaryllifolius on neutrophil, red blood cell, and platelet functions.

Materials and methods: Boiled herb extracts of Phyllanthus amarus Schum&Thorn, Phyllanthus acidus (L.) Skeels, and Pandanus amaryllifolius was used at the final concentrations of 100, 200 and 400 μg/mL. The oxidative stress levels in red blood cells, phagocytic function of neutrophils and platelet function were determined using malondialdehyde assay, phagocytic assay, and platelet aggregation assay, respectively.

Results: All concentrations of boiled herb extracts could significantly inhibited collagen-activated platelet aggregation at p<0.05 when compared to the control. The boiled herb extract (200 and 400 μg/mL) showed significantly increased phagocytic function (p<0.05). However, level of oxidative stress slightly decrease (not significant when compared to the control) after boiled herb extract treatment.

Conclusions: Folk wisdom boiled herb extract, Phyllanthus amarus Schum&Thorn, Phyllanthus acidus (L.) Skeels, and Pandanus amaryllifolius extract could enhance phagocytic cell function and inhibit platelet aggregation.

Bull Chiang Mai Assoc Med Sci 2015; 48(2): 144-153


Keywords


Folk wisdom boiled herb; hematopoietic cells; oxidative stress; phagocytosis; platelet aggregation

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References


Nilesh KJ, Santram L, Avijeet J, Alok N, Abhay KS. Effect of Phyllanthus acidus (L.) Skeels fruit on carbon tetrachloride-induced acute oxidative damage in livers of rats and mice. J Chin Integr Med. 2011; 9: 49-56.

Johnson M, Alias A. Somoclonal variation studies on Phyllanthus amarus schum & Thonn. Int J Biochem. 2007; 5(4): 240-5.

Dhar ML, Dhar MM, Dhawan BN, Mehrotra BN, Ray C. Screening of Indian plants for biological activity: part I. Indain J Exp Biol .1968; 6: 232-47.

Ooi LS, Sun SS, Ooi VE. Purification and characterization of a new antiviral protein from the leaves of Pandanus amaryllifolius (Pandanaceae). Int J Biochem Cell Biol. 2004; 36(8): 1440-6.

Nor FM, Mohamed S, Idris NA, Ismail R. Antioxidative properties of Pandanus amaryllifolius leaf extracts in accelerated oxidation and deep frying studies. Food Chem. 2008; 110(2): 319-27.

Chirico S. High-performance liquid chromatography-based thiobarbituric acid tests. Methods Enzymol. 1994; 233: 314-8.

Wardle EN. Assessment of neutrophil function-II. Laboratory tests of neutrophil function. Postgrad Med J. 1986; 62(734): 1089-92.

Bednar B, Condra C, Gould RJ, Connolly TM. Platelet aggregation monitored in a 96 well microplate reader is useful for evaluation of platelet agonists and antagonists. Thromb Res. 1995; 77(5): 453-63.

Amic D, Davidovic D, Beslo D and Trinajstic N. Structure-radical scavenging activity relationships of flavonoids. Croat Chem Acta. 2003; 76: 55-61.

Jimtaisong A, Krisdaphong P. Antioxidant activity of Pandanus amaryllifolius leaf and root extract and its application in topical emulsion. Trop J Pharm Res. 2013; 12(3): 425-31.

Jain NK, Singhai AK. Protective effects of Phyllanthus acidus (L.) Skeels leaf extracts on acetaminophen and thioacetamide induced hepatic injuries in Wistar rats. Asian Pac J Trop Med. 2011; 4(6): 470-4.

Nor FM, Mohamed S, Idris NA, Ismail R. Antioxidative properties of Pandanus amaryllifolius leaf extracts in accelerated oxidation and deep frying studies. Food Chem. 2008; 110(2): 319-27.

Harikumar K, Kuttan R. An extract of Phyllanthus amarus protects mouse chromosomes and intestine from radiation induced damages. J Radiat Res. 2007; 48: 469-76.

Kobzar G, Mardla V, Samel N. Effects of α-tocopherol, l-arginine, and quercetin on aggregation of human platelets. Nutr Res. 2005; 25(6): 569-75.

Furie B, Furie BC. Cancer-associated thrombosis. Blood Cell Mol Dis. 2006; 36(2): 177-81.

Setiabudy R, Wahidiyat PA, Setiawan L. Platelet aggregation and activation in thalassemia major patients in Indonesia. Clin Appl Thromb Hemost. 2008; 14(3): 346-51.

Lopez E, Berna-Erro A, Hernandez-Cruz JM, Salido GM, Redondo PC, Rosado JA. Immunophilins are Involved in the Altered Platelet Aggregation Observed in patients with Type 2 Diabetes Mellitus. Curr Med Chem. 2013; 20(14): 1912-21.

Franchini M, Targher G, Montagnana M, Lippi G. The metabolic syndrome and the risk of arterial and venous thrombosis. Thromb Res. 2008; 122(6): 727-35.

Davi G, Patrono C. Platelet activation and atherothrombosis. N Engl J Med. 2007; 357: 2482-94.

Antara S, Amla B. The study of in vitro and in vivo antioxidant activity and total phenolic content of Phyllanthus amarus Schum&Thorn: A medicinally important plant. Int J Pharm Pharm Sci. 2013; 5(3): 942-7.

Tapas, AR, Sakarkar, DM, Kakde, RB. Flavonoids as nutraceuticals: A Review. Trop J Pharm Res. 2008; 7: 1089-99.

Jain NK, Singhai AK. Protective effects of Phyllanthus acidus (L.) Skeels leaf extracts on acetaminophen and thioacetamide induced hepatic injuries in Wistar rats. Asian Pac J Trop Med. 2011; 4: 470-4.

Middleton E Jr. Effects of flavonoids on immune and inflammatory cell functions. Biochem Pharmacol. 1992; 43(6): 1167-79.


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